I also installed Kali rolling 2016.2 x64 and have no sound. I am on a Lenovo T420 laptop.

i had also the same problem i tried using these commands in terminal.
pulseaudio -D
pulseaudio -D --system
It worked for me...

What does "systemctl --user status pulseaudio" return when you have no sound?

I recall this happening once to me. However, it was isolated to root account only. Might I suggest trying a different user-account. iirc, the only fix was using alsa-mixer over pulse-audio.

every time I logged into my root I have to enter
pulseaudio -D
and the problem fixes.

"pulseaudio -D"
Fixes the error. It worked for me.

This is what "systemctl --user status pulseaudio" returns when I have no sound.

root@kali:~# systemctl --user status pulseaudio
● pulseaudio.service - Sound Service
Loaded: loaded (/usr/lib/systemd/user/pulseaudio.service; disabled; vendor pr
Active: inactive (dead)
lines 1-3/3 (END)...skipping...
● pulseaudio.service - Sound Service
Loaded: loaded (/usr/lib/systemd/user/pulseaudio.service; disabled; vendor preset: enabled)
Active: inactive (dead)

More info after pulseaudio has been initiated...

root@kali:~#
root@kali:~#
root@kali:~# pulseaudio
W: [pulseaudio] main.c: This program is not intended to be run as root (unless --system is specified).
E: [pulseaudio] bluez5-util.c: GetManagedObjects() failed: org.freedesktop.systemd1.NoSuchUnit: Unit dbus-org.bluez.service not found.
root@kali:~#
root@kali:~#
root@kali:~# pulseaudio -D --system
W: [pulseaudio] main.c: Running in system mode, but --disallow-exit not set.
W: [pulseaudio] main.c: Running in system mode, but --disallow-module-loading not set.
N: [pulseaudio] main.c: Running in system mode, forcibly disabling SHM mode.
N: [pulseaudio] main.c: Running in system mode, forcibly disabling exit idle time.
root@kali:~#
root@kali:~#
root@kali:~# systemctl --user status pulseaudio
● pulseaudio.service - Sound Service
Loaded: loaded (/usr/lib/systemd/user/pulseaudio.service; disabled; vendor preset: enabled)
Active: inactive (dead)
root@kali:~#
root@kali:~#

I have tried all suggested solutions above but none of them were a permanent fix. I have the same issue after reboot.

I did not have any sound issue with the previous Kali rolling 2016.1 x64 or Kali 1.0.0 builds on the same laptop I am currently using - Lenovo T420 Laptop.

The permanent fix should be "systemctl --user enable pulseaudio && systemctl --user start pulseaudio".

That said, the service should be enabled by default in the live image and in any installed system since we configure that in the build process (it was an older bug, 0002392). If that's no longer the case, then there's something disabling it upon us.

I used "systemctl --user enable pulseaudio && systemctl --user start pulseaudio" and it worked. Pusleaudio starts up after reboot. Thx.

Command pulseaudio -D solved the problem...
Thanx... :)

So actually, the code that enables pulseaudio is still here in the live-build hook but it fails with this error message:
Assertion 'config_path' failed at ../src/shared/install.c:1706, function install_context_apply(). Aborting.
Aborted

I reported this to the upstream systemd developers here:
https://github.com/systemd/systemd/issues/5260

I have pushed a work-around to the live-build-config which lets systemctl do its job until we have a fixed version of systemd.

The next weekly and daily images should again have sound by default.